Tubulin beta, NT (Beta-tubulin, Tubulin beta Chain)

Tubulin beta is a 50kD globular protein and major constituent of microtubules within the cytoskeleton, involved in meiosis, mitosis, intracellular transport and maintaining cell shape.\n\nThe evolutionarily conserved heterodimer tubulin, made up of a tubulin alpha and beta chain, binds two molecules of GTP, one at a non-exchangeable site on the alpha chain and one at an exchangeable site on the beta chain. Only the beta chain possesses GTPase activity however, hydrolysing GTP to GDP, and influencing the stability of the dimer within the microtubule.\n\nT9155-01Q is reported to partially inhibit microtubule assembly in vitro (2).\n\nApplications:\nSuitable for use in ELISA, Immunohistochemistry (frozen), and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9155-01Q

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Prc

Form

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

P Type

Mab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgM

References

1. Krauhs, E. et al. (1981) Complete amino acid sequence of beta-tubulin from porcine brain. P.N.A.S. 78: 4156-4160. 2. Draber, P. et al. (1990) Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 262: 209-211.

Additional Info

Recognizes an epitope within the N-terminal region of porcine tubulin beta. Species Crossreactivity: human, plants, mouse.