Tubulin, beta (Recombinant) (Tubulin beta Chain, Tubulin beta-5 chain)

anti-beta-Tubulin, monoclonal antibody (recombinant)(S11B) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.\n \n\n\nApplications:\nSuitable for use in ELISA, Immunocytochemistry, Western Blot\n\nRecommended Dilution:\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

045380

Size

100ug

Applications

ELISA, ICC, WB

Form

Supplied as a liquid in PBS, 10% glycerol, 0.02% sodium azide.

P Type

Mab

Purity

Purified from HEK 293 cell culture supernatant (>95% (SDS-PAGE).

Isotype

IgG2,l

References

Product Reference: \n?Recombinant antibodies selected against subcellular fractions to track endogenous protein dynamics in vivo: C. Nizak, et al.; Traffic 7, 739 (2003)\n?

Additional Info

Recognizes human, mouse, rat, pig, drosophila and monkey beta-tubulin.

Alternative Names

Tubulin beta Chain, Tubulin beta-5 chain