Tubulin is a major cytoskeleton component that has five distinct forms. a and beta Tubulins form heterodimers which multimerize to form a microtubule filament. There are five beta Tubulin isoforms (beta1, beta2, beta3, beta4A and beta4B) that are expressed in mammalian tissues. beta1 and beta4 are present throughout the cytosol, beta2 is present in the nuclei and nucleoplasm, and beta3 is a neuron-specific cytoskeletal protein.
Applications:
Suitable for use in ELISA, Western Blot and Immunofluorescence. Other applications not tested.
Recommended Dilution:
Western Blot: 1:5,000
Immunofluorescence: 1:1,000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.