logo
logo
TUC-4 Protein (TOAD, Turned On After Division, Ulip/CRMP-4, DRP-3, Dihydropyrimidinase-like 3)

Cat no: T9157

TUC-4 Protein (TOAD, Turned On After Division, Ulip/CRMP-4, DRP-3, Dihydropyrimidinase-like 3)

TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (4). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (2). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (3). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (4). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (2). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (3) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR).\n\nApplications: \nSuitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:2500-1:25,000 using akaline phosphatase\nImmunohistochemistry: 1:1000-1:5000 using PFA (DAB detection).\nImmunocytochemistry: 1:1000-5000 (DAB detection).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9157

Size

100ul

Applications

ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Fel, NHP

Form

Supplied as a liquid, 0.2% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Liu, S., et al., J. Neuroscience 23: 732-736 (2003). 2. Minturn, J.E., et al., J. Neuroscience 15: 6757-6766 (1995). 3. Minturn, J.E., et al., J. Comparative Neurology 355: 369-379 (1995). 4. Quinn, C.C., et al., J. Neurobiology 41: 158-164 (1999). 5. Grass, D., et al., J. Neuroscience 24: 1358-1365 (2004). 6. Bauer, S., et al., Neuroscience 130: 75-90 (2005). 7. Lin, C., et al., PNAS 102: 14,877-14,882 (2005). 8. Li, J., et al., J. Neuroscience 26: 7839-7848 (2006).

Additional Info

Recognizes human TUC-4 protein (ULIP1/CRMP4). Reported to label early postmitotic neurons in prenatal rat brain. Not reactive with the proteins TUC-1 (ULIP3/CRMP1), TUC-2 (ULIP2/CRMP2) or TUC-3 (ULIP4/CRMP3). Does not label neural progenitor or radial glia. Species Crossreactivity: Rat, mouse, feline and monkey.

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

Use promo code EASY3 to receive 13% off and FREE shipping!Experience a new dimension of electronic pipetting with the NEW Easypet 3 pipette controller. The...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...