Human b-Tubulin 3 is a 50,432 dalton structural protein (450 amino acid) expressed in neurons of the PNS and CNS. It contributes to microtubule stability in neuronal cell bodies and axons, and plays a role in axonal transport.
Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1:250-1:500
Immunohistochemistry: 1:100-1:200
Immunocytochemistry: 1:100-1:200
Optimal dilutions to be determined by the researcher.
Recommended Secondary Antibodies:
I1906: IgY, Chicken Pab Rb x Ch
I1906-10: IgY, Chicken (HRP) Rb x Ch
I1906-12: IgY, Chicken (FITC) Rb x Ch
I1906-14: IgY, Chicken (AP) Rb x Ch
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.