Tumor Necrosis Factor alpha, Low Endotoxin (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, Cachectin, TNF, TNFA, TNFSF2)

TNF alpha is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Functional Assays. Other applications not tested. \n\nRecommended Dilution:\nFlow Cytomtery: 1:50-1:100; 10ul labels 10e6 cells in 100ul\nELISA: 1-10ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9160-13K

Size

500ug

Applications

ELISA, FC, IHC

Hosts

Rat

Reactivities

Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Abrams, J. S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples Immunol. Rev. 127: 5-24. 2. Hostager, B. et al. (2002) Role of TNF receptor-associated factor 2 in the activation of IgM secretion by CD40 and CD120b. J. Immunol. 168:3318-3322. 3. Ross, S. et al. (1997) Suppression of TNF-alpha expression, inhibition of Th1 activity, and amelioration of collagen-induced arthritis by rolipram. J. Immunol. 159:6253-6259. 4. Scanga, C. et al. (1999) Reactivation of latent tuberculosis : variations on the Cornell murine model. Infect. Immun. 67:4531-4538. 5. Yang, J. et al. (1995) Involvement of natural killer cells in nitric oxide production by spleen cells after stimulation with Mycobacterium bovis BCG. Study of the mechanism of the different abilities of viable and killed BCG. J. Immunol. 155:5728-5735.

Additional Info

Recognizes mouse TNF, alpha. Species Crossreactivity: rat.