Tumor Necrosis Factor alpha, Recombinant Human (TNF alpha, TNFa)

Human Tumor necrosis factor (TNF- alpha), also known as Cachectin, is a cytokine composed of 157 aa residues, having a molecular weight of 17kD (monomer). There is 79% homology between human and murine TNF- alpha, including conserved cysteine residues which form an intrachain disulfide bridge. Biological effects of this cytokine include antitumor activity in vivo and in vitro, activation of polymorphonuclear leukocytes, antiviral activity, and the induction of the release if Interleukin-1 or colony stimulating factors from a variety of sources. \n\nSource:\nyeast\n\nBiological Activity: \nThe cell cytolytic activity is determined in the L929 cells (3 x 104 cells/assay) and it is 2-4 x 108 U/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-18E

Size

10ug

Form

Supplied as a lyophilized powder in PBS, 0.02M Tris-HCl/ 0.15M NaCl, pH 8.0, 0.1% sodium azide.

Purity

Purified. (same/more than) 95% pure by SDS gel electrophoresis.

References

1. Feinstein, R., Kanety, H., Papa, M.Z., lunenfeld, B., and Karasik, A., (1993) The Journal of Biological Chemistry, Vol 268, No. 35, December, 26055-26058 2. Kalthoff, H., Roeder, C., Brockhaus, M., Thiele, H.G., and Schmiegel, W., (1993) The Journal of Biological Chemistry, Vol 268, No. 4, February, 2762-2766 3. Chaturvedi, M.M. et al. (1994). J. Biol Chem. 269, 14575-14583.