Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Tumor necrosis factor alpha (TNF-a), also called cachectin, consists of 158 amino acids. TNF-a is a 17.5kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-a is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Additional amino acid, methionine, was attached at N-terminus of the protein.\n\nAmino Acid Sequence:\nMVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY \nSQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY \nLGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL\n\nBiological Activity: \nMeasured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.16ng/ml.\n\nActivity Assay:\nL929 cytotoxicity assay using actinomycin D\nCell Number: 2 x 10,000 cells/well\nIncubation: 24hr after sample treatment\nAssay Media: 5% FBS media\nCytokine Concentration: 12 pg/ml - 25 ng/ml\nDetection method: MTT assay\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-03H

Size

100ug

Form

Supplied as a liquid in PBS, pH 7.4

Purity

> 95% by SDS PAGE. Purified by using conventional chromatography techniques. Endotoxin: (same/less than)1EU/ug protein (LAL method)

References

1. Ruddle, N.H., et al., Curr. Opin. Immunol. 4: 327-332 (1992). 2. Chicoine, M.R., et al., J. Neurooncol. 35: 249-257 (1997). 3. Wingfield, P., et al., FEBS Lett. 211: 179-184 (1987). 4. Tikhonov, R.V., et al., Russ. J. Bioorg. Chem. 22: 141-144 (1996). 5. Barbara, J.A.J., et al., Proc. Natl. Acad. Sci. USA 83: 4533-4537 (1986).