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Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Cat no: T9160-10D

Tumor Necrosis Factor alpha, Recombinant, Human (TNFa)

Recombinant Human Tumor Necrosis Factor alpha is a single, non-glycosylated, polypeptide chain containing 158aa and having a molecular weight of 17.5kD. The sequence of the first five N-terminal amino acids was determined to be Met-Val-Arg-Ser-Ser. \n\nBiological Activity:\nThe ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is a specific activity of (same/more than)5x10e7 IU/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with ddH2O, 0.1% HSA or BSA. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-10D

Size

50ug

Form

Supplied as a lyophilized powder from 20mM PBS, pH 7.2, 100mM sodium chloride. Reconstitute with sterile ddH2O to (same/more than)100ug/ml.

Purity

(same/more than)95% by RP-HPLC and SDS-PAGE

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