Tumor Necrosis Factor alpha, Recombinant, Mouse (TNFa)

Recombinant murine TNF-alpha produced in S. lividians is a single, non-glycosylated, polypeptide chain containing 156 amino acids and having a molecular mass of 17301.32D.\n\nBiological Activity: rmTNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is less then 0.05ng/ml, corresponding to a Specific Activity of 9x10e8 IU/mg.\n\nAA Sequence:\nMLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-15C

Size

5ug

Form

Supplied as a lyophilized powder in 50mM Tris-HCL pH-8 and 20mM NaCL. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

(same/more than) 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Tumor necrosis factor-alpha mediates oligodendrocyte death and delayed retinal ganglion cell loss in a mouse model of glaucoma. J Neurosci 2006 Dec 6;26(49):12633-41\n2. Endogenous prostaglandins E2 and F 2alpha serve as an anti-apoptotic factor against apoptosis induced by tumor necrosis factor-alpha in mouse 3T3-L1 preadipocytes. Biosci Biotechnol Biochem 2006 Sep;70(9):2145-53\n3. Toxoplasma gondii inhibits toll-like receptor 4 ligand-induced mobilization of intracellular tumor necrosis factor alpha to the surface of mouse peritoneal neutrophils. Infect Immun 2006 Jul;74(7):4274-81\n4. Ursolic acid enhances cyclooxygenases and tumor necrosis factor-alpha expression in mouse skin. Biosci Biotechnol Biochem 2006 Apr;70(4):1033-7\n5. The role of tumor necrosis factor alpha and the peroxisome proliferator-activated receptor alpha in modulating the effects of fumonisin in mouse liver. Toxicology 2006 May 15;222(3):165-74\n6. Acute p38-mediated modulation of tetrodotoxin-resistant sodium channels in mouse sensory neurons by tumor necrosis factor-alpha. J Neurosci 2006 Jan 4;26(1):246-55