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Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

Cat no: T9160-02A

Tumor Necrosis Factor alpha, Recombinant, Rat (TNFa)

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. \n\nMolecular Weight: 17.3kD (157 Amino acid sequence)\n\nEndotoxin Level: <0.1ng/ug \n\nBiological Properties: Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells-this batch demonstrated an ED50 of < 0.5ng/ml.\n\nApplications:\nOptimal working dilutions to be determined by researcher.

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SPECIFICATIONS

Catalog Number

T9160-02A

Size

20ug

Reactivities

Rat

Form

Supplied as a lyophilized powder in PBS. Reconstitute with 40ul distilled water.

Purity

Highly Purified (> 98% by SDS PAGE and HPLC).

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