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Tumor Necrosis Factor Alpha (TNF-alpha) (FITC)

Tumor Necrosis Factor Alpha (TNF-alpha) (FITC)

Cat no: T9160-15F

Supplier: United States Biological
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TNF-alpha can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII). For this purpose we recommend to use this antibody in combination with the anti-TNF-alpha antibody, which recognizes soluble, membrane and receptor bound TNF-alpha. Applications: Suitable for use in Flow Cytometry, Western Blot, and immuno assays. Other applications not tested. Recommended Dilutions: Functional Assays: 1:50 Western Blot: 1:50 Optimal ddilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
Catalogue number: T9160-15F
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide, Labeled with fluorescein isothiocyanate (FITC).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: TNF-a, cachetin
References: 1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243
Additional info: Recognizes the free soluble (17kD) and membrane (26kD) human TNF-alpha. The antibody inhibits the biological activity of both forms. It does not react with receptor bound TNF-alpha.

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