Home  >  Products  >  Tumor Necrosis Factor alpha (TNF alpha, TNF-alpha, TNF a, TNFa, TNF-a, Cachectin, Differentiation Inducing Factor, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF Superfamily Member 2, TNFSF2)
Tumor Necrosis Factor alpha (TNF alpha, TNF-alpha, TNF a, TNFa, TNF-a, Cachectin, Differentiation Inducing Factor, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF Superfamily Member 2, TNFSF2)

Tumor Necrosis Factor alpha (TNF alpha, TNF-alpha, TNF a, TNFa, TNF-a, Cachectin, Differentiation Inducing Factor, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF Superfamily Member 2, TNFSF2)

Cat no: T9160-03R

Supplier: United States Biological
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Tumor necrosis factor-alpha (TNF-alpha), a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro- organisms and by various cytokines but it also induces on its turn the production of many cytokines. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors I and II. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-receptors leading to receptor crosslinking. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen and paraffin): 1:50 Western Blot: 1:50 Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9160-03R
Reactivities: Human, Mouse, Guinea Pig, Non-Human Primate
Hosts: Mouse
Applications: ELISA, Flow Cytometry, Immunohistochemistry, Western Blot
Size: 1ml
Form: Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
Alternative names: APC1, Macrophage Cytotoxic Factor, MCF, Necrosin, TNF Macrophage-derived, TNF Monocyte-derived, Tumor Necrosis Factor Precursor
References: 1. Gerspach, J. et al: Microsc Res Tech 2000, 50:243 2. Bradding, P. et al: Am J. Respir Cell Mol Biol 1994, 10:471 3. Bradding, P et al: Clin Exp Allergy 1994, 25:406 4. Laan van der, N et al: Arch Dermatol Res 2001, 293:226.
Additional info: Recognizes native and recombinant human TNF-a. Species Crossreactivity: mouse, guinea pig and rhesus monkey. Lacks cross reactivity with TNF-b or lymphotoxin. Reacts with free soluble (17kD) and membrane (26kD) human TNF-a. Does not react with receptor bound TNF-a.

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