Tumor Necrosis Factor alpha (TNF alpha, TNF-alpha, TNFalpha, TNFa, TNF-a, Cachectin, Differentiation Inducing Factor, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF Superfamily Member 2, TNFSF2)

TNF-alpha is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts.\n\nTNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis.\n\nApplications:\nSuitable for use in ELISA, Functional Assays, and Western Blot. Other applications not tested. \n\nRecommended Dilution:\nELISA: 0.5ug/ml\nWestern Blot: 0.1-0.2ug/ml\nFunctional Assays: 0.01-0.015ug/ml\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-03E2

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Van Horssen, R. et al. (2006) TNF-alpha in cancer treatment: molecular insights, antitumor effects, and clinical utility. The Oncologist. 11: 397-408.

Additional Info

Recognizes mouse TNF-alpha.

Alternative Names

MGC151434, Tnfsf1a