Home  >  Products  >  Tumor Necrosis Factor alpha (TNF-alpha, TNFa, TNF-a, TNF, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)
Tumor Necrosis Factor alpha (TNF-alpha, TNFa, TNF-a, TNF, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

Tumor Necrosis Factor alpha (TNF-alpha, TNFa, TNF-a, TNF, Cachectin, DIF, Tumor Necrosis Factor Ligand Superfamily Member 2, TNFSF2)

Cat no: T9160-36T

Supplier: United States Biological
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
Read More on Supplier Website
TNF-a, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1,2). Membrane-bound TNF-a is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-a are biologically active. TNF-a is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-a is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, ERK (p44/42), p38 MAPK, and NF-kB) promotes the survival of cells, while TNF-a-mediated activation of caspase-8 leads to programmed cell death (1,2). TNF-a plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-a in autoimmunity is underscored by blocking TNF-a action to treat rheumatoid arthritis and Crohn's disease (1,2,4). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9160-36T
Reactivities: Human
Hosts: Rabbit
Applications: Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1.Aggarwal, B.B. (2003) Nat Rev Immunol 3, 745-56. 2.Hehlgans, T. and Pfeffer, K. (2005) Immunology 115, 1-20. 3.Lin, P.L. et al. (2007) J Investig Dermatol Symp Proc 12, 22-5. 4.Brennan, F.M. and McInnes, I.B. (2008) J Clin Invest 118, 3537-45.
Additional info: Recognizes endogenous levels of total full length and secreted human TNF-a. It can detect up to 10pg of recombinant TNF-a by western blot.

Get Quote

  • Best Price Guaranteed
  • Quick Response Time
  • Exclusive Promotions
Enquiry_down_arrow
United States Biological
Get a Quote Direct from
United States Biological

By submitting this form you agree to your details being passed to United States Biological for the purpose of generating the best quote*
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave