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Tumor Necrosis Factor alpha (TNFa, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFSF2) (PE)

Tumor Necrosis Factor alpha (TNFa, Cachectin, TNF-alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFSF2) (PE)

Cat no: T9160-29H

Supplier: United States Biological
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Tumor Necrosis Factor alpha (TNF-a) is a potent immunomodulator and proinflammatory cytokine. The secreted form of TNF-a is 17kD while the membrane form (mTNF-a) has a molecular weight of 26kD. Secreted TNF-a appears to be generated from the precursor mTNF-a. 1 Membrane TNF-a has been described on the surface of monocytes and activated T lymphocytes.This anti-TNF-a monoclonal antibody recognizes mTNF-a transfected into a cell line, and is designed for use as a flow cytometry reagent. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human TNF-a. Storage and Stability: May be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4 degrees C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
Catalogue number: T9160-29H
Reactivities: Human
Hosts: Mouse
Applications: Flow Cytometry
Size: 50test
Form: Supplied as a liquid in 0.5ml saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).
P type: Mab
Isotype: IgG1
Purity: Purified by Protein G affinity chromatography.
References: 1. Kriegler, M. et al. (1988) Cell 53:45. 2. Kinkhabwala, M. et al. (1990) J. Exp. Med. 171:941. 3. Scallon, B.J. et al. (1995) Cytokine 7:251.
Additional info: Recognizes human TNF-a.

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