Tumor Necrosis Factor alpha (TNFa)

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. \n\nApplications:\nUseful for inhibition of the biological activity of TNF-a. It has been tested in cytotoxicity assays as well as in assays with activation of endothelial cells and found to be a powerful antibody. It is can be used to discriminate between TNF-a and TNF-b or lymphotoxin. Furthermore the antibody is useful for immunoassays, Immunoprecipitation, Flow Cytometry and Western Blot.. The antibody stains TNF-a in frozen sections of inflamed tissues.\n\nRecommended Dilution:\nImmunohistology: 1:10\nFlow Cytometry: 1:10\nWestern Blotting: 1:10; For neutralization of biological activity in vitro dilutions have to be made according to the amount of TNF-a to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-04J

Size

100ug

Applications

FC, IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243 2. Limb, GA et al; Distribution of TNF-a and its reactive vascular adhesion molecules in fibrovascular membranes of proliferative diabetic retinopathy. Br J Ophthalmol 1996, 80: 168.

Additional Info

Reacts with human native and recombinant TNF-a as assessed by ELISA. Inhibits the biological activity of human native and recombinant TNF-a as determined with L929 cells in a cytotoxicity assay. Crossreacts with rhesus and cynomolgus natural TNF-a and lacks crossreactivity with human lymphotoxin.