Tumor Necrosis Factor alpha (TNFa)

Tumor Necrosis Factor-alpha (TNF-alpha) is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF superfamily of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also plays a role in the induction of septic shock, autoimmune diseases, rheumatoid arthritis, inflammation and diabetes.\n\nApplications:\nSuitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistology (frozen and paraffin): 1:50. Non-specific background staining is seen in connective tissue. \nFlow Cytometry: 1:50\nWestern Blot: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9160-04K

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

Reactivities

Hum, Mouse, GP, NHP

Form

Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Gerspach, J et al., (2000) 50:243. 2. Bradding, P et al., (1994) Am J Respir Cell Mol Biol, 10:471. 3. Radding, P et al., (1994) Clin Exp Allergy 25:406. 4. Laan van der, N et al., (2001) Arch Dermatol Res 293: 226.

Additional Info

Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not crossreact with TNF-beta, lymphotoxin or receptor bound TNF-alpha. Species Crossreactivity: mouse, guinea pig and rhesus monkey.