Tumor Necrosis Factor alpha (TNFa)

Applications:\nUseful for inhibition of the biological activity of TNF-alpha. The antibody is also useful for Immunoprecipitation, immunoassays, Flow Cytometry and Western Blot.\n\nRecommended Dilution:\nFlow Cytometry: 1:10\nWestern Blot: 1:10\nFor neutralization of biological activity in vitro dilutions have to be made according to the amounts of TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-04P

Size

100ug

Applications

FC, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Gerspach, J et al; Detection of membrane-bound Tumor Necrosis Factor (TNF): an analysis of TNF-specific reagents. Microsc Res Tech 2000, 50: 243.

Additional Info

Reacts with free soluble (17kD) and membrane (26kD) human TNF-alpha. Inhibits the biological activity of both forms. Does not react with receptor bound TNF-alpha. It can be a useful tool to discriminate between the membrane form of TNF expressed on producer cells and the proteolytically cleaved, soluble TNF-alpha bound to its cognate cell membrane receptors (TNF-RI and TNF-RII).