Tumor Necrosis Factor alpha (TNFa)

Mixture of two monoclonal antibodies provided at a total concentration of 2.5mg/ml). Please note that this lot is provided at an increased concentration due to the higher recommended coating concentration. \n\nTumor Necrosis Factor alpha (TNF-alpha) is a cytokine which has diverse immunomodulatory, anti-tumor and toxic effects. TNF-alpha has been detected in diverse inflammatory status and appears to be a critical mediator in the lethality of septic shock. Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. The antibody shows neutralizing activity.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nELISA: Capture\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nNSO myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9160-22G

Size

500ug

Applications

ELISA

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a+IgG1

References

1. Braun, M.C., J. He, C.-Y. Wu, and B.L. Kelsall (1999) Cholera toxin suppresses interleukin (IL)-12 production and IL-12 receptor b1 and b2 chain expression. J. Exp. Med. 189:541-552.\n\n2. Gee, K., W. Lim, W. Ma, D. Nandan, F. Diaz-Mitoma, M. Kozlowski, and A. Kumar (2002) Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: Distinct involvement of c-Jun Nterminal kinase in LPS-induced CD44 expression. J. Immunol. 169 (10):5660-5672.

Additional Info

Recognizes natural and recombinant soluble human TNF-alpha.