Tumor necrosis factor-alpha (TNFA) also kowns as TNFR1, It is a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. TNFR1 maps to 12p13. TNFR1 signaling is also known to activate the transcription factor NF-kappa B and promote survival. Tumor necrosis factor, alpha-induced protein 1 (endothelial), also known as TNFAIP1, is a human gene.The gene, present in single copy, was located in the 17q22-q23 region. This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. The protein is involved in the primary response of the endothelium to TNF.
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions:
Western Blot: 1ug/ml. The detection limit is ~2.5ng/lane under non-reducing and reducing conditions.
Immunohistochemistry (Paraffin): 1ug/ml
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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