Tumor Necrosis Factor beta, Human (TNFb) BioAssay(TM) ELISA Kit

For the quantitative determination of human Tumor Necrosis Factor-b (TNF-b) concentrations in serum, plasma, cell culture supernatant, and other biological fluids.\n\nThis assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF-b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-b present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody-enzyme specific for TNF-b is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-b bound in the initial step. The color development is stopped and the intensity of the color is measured.\nIn order to measure the concentration of TNF-b in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-b concentration (pg/ml). The concentration of TNF-b in the samples is then determined by comparing the O.D. of the samples to the standard curve. This immunoassay is calibrated against natural human TNF-b (NIBSC/WHO First International Standard 87/640).\n\nKit Components:\nT9160-40C: TNFb Microtiter Plate, 1x96wells (Pre-coated with a monoclonal anti-human TNFb)\nT9160-40D: Pab (Biotin) Conjugate, 1x11ml (Anti-human TNFb labeled with Biotin)\nT9160-40E: Avidin (HRP) Conjugate, 1x14ml \nT9160-40F: TNFb Standard 0.8ng/vial, 2x1vial\nT9160-40G: Serum Assay Buffer, 1x6ml\nT9160-40H: Calibrator Diluent I, 1x22ml\nT9160-40J: Calibrator Diluent II, 1x22ml\nT9160-40K: Wash Buffer, 20X, 1x60ml\nT9160-40L: Substrate A, 1x10ml\nT9160-40M: Substrate B, 1x10ml\nT9160-4N: Stop Solution, 1x14ml\n\nSensitivity: \nCalibrator Diluent I: 22pg/ml\nCalibrator Diluent II: 11pg/ml\n\nAssay Range: 125-8000pg/ml\n\nStorage and Stability: \nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9160-40A

Size

96Tests

Applications

ELISA

References

1. Aggarwal, B.B., Vilcek, J. (eds) (1992) Tumor Necrosis Factor: Structure, Function and Mechanism of Action, Marcel Dekker, New York. 2. Eck, M.J., et al. (1992) J. Biol. Chem. 267:2119. 3. Gray, P.W., et al. (1984) Nature 312:721. 4. Grom, A. A., (1996). Arthritis Rheum. 39: 1703-1710. 5. Ishibashi, K., et al. (1991). Blood 77: 2451-2455. 6. Jeffes, E. W., (1989). Arthritis Rheum. 32: 1148-1152. 7. Laham, N., et al. (1997). J. Reprod. Immuno. 33: 53-69. 8. Lee KM., et al (2005) Breast Cancer Res Treat. 90:149-55. 9. Noguchi, M., et al. (1998). Gut 43: 203-209. 10. Ruddle, N.H. (1994) The Cytokine Handbook, A. Thomson ed., Academic Press, New York, p. 305. 11. Schoenfeld, H.J. et al. (1991) J. Biol. Chem. 266:3863. 12. Selmaj, k., et al. (1997) J. Clin. Invest. 87: 949-954. 13. Selmaj, K., et al. (1998) Ann. Neurol. 30: 694-700. 14. Vilcek, J. and T.H. Lee (1991) J. Biol. Chem. 266:7313. 15. Warzocha, K., (1998). Br. J. Cancer 77: 2357-2362. 16. YuneTY.,etal,J Neurotrauma.(2004) 21: 1778-94.