Tumor Necrosis Factor beta (TNF beta, TNFbeta, TNF-beta, TNF b, TNFb, Coley

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. \n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Secreted\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9160-43D

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

PBS with 0.01% sodium azide

P Type

Pab

Purity

Purified

Isotype

IgG

References

Granger GA & Kolb WP Lymphocyte in vitro cytotoxicity: mechanisms of immune and non-immune small lymphocyte mediated target L cell destruction. J Immunol 101:111-20 (1968). <PUBMED:5690881>Williams TW & Granger GA Lymphocyte in vitro cytotoxicity: lymphotoxins of several mammalian species. Nature 219:1076-7 (1968). <PUBMED:5673378>Ruddle NH & Waksman BH Cytotoxic effect of lymphocyte-antigen interaction in delayed hypersensitivity. Science 157:1060-2 (1967). <PUBMED:6036235>

Additional Info

Recognizes human TNF-beta, and demonstrates neutralizing activity in functional assays. Species Crossreactivity: Crossreacts with Human.