Tumor Necrosis Factor Receptor 1, p55 (TNFR 1, CD120a, MGC19588, p55, p55 R, TNFR55, Tumor Necrosis Factor alpha Receptor, TNF Binding Protein 1, TBP1, TNF Receptor Superfamily Member 1A, TNFRSF1a) (FITC)

The immunoregulatory cytokine tumor necrosis factor alpha (TNFa) recognizes two cell surface receptors. TNF-R1 (also known as CD120a) has an apparent molecular mass of 55/60kD and is constitutively expressed on all nucleated cells, whereas TNF R2 (also known as CD120b) with an apparent molecular mass of 75/80kD, is restricted to hematopoietic cells. TNF-R2 directs TNFa mediated activation and proliferation of T lymphocytes. An autocrine role for TNF and TNF-R2 is implicated in hematopoietic malignancies, and the TNF-R2 locus is a translocation target in several leukemias, suggesting a role for TNF-R2 in the development of certain leukemias.\n\nApplications:\nSuitable for use in Flow Cytometry. Other applications have not been tested.\n\nRecommended Dilutions:\nFlow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood.\nOptimal working dilutions to be determined by researcher.\n\nRecommended Negative Control: \nI1904-83Y1: IgG2a Negative Control (FITC)\n\nHybridoma: \nSpleen cells from immunized BALB/c mice fused with mouse NSO myeloma cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

T9161-06C

Size

100ug

Applications

FC

Hosts

Mouse

Reactivities

Hum, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with FITC.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2a

References

1. Thoma B. et al. 1990. J. Exp. Med. 172:1019-1023. 2. Menegazzi R. et al. 1994. Blood. 84: 287-293. 3. Dri P. et al. 1999. J. Immunol. 162:460-466. 4. Kohrgruber N. et al. 1999. J Immunol. 163:3250-3259. 5. Weigart N. et al. 1996. Gasteroenterology. 110:147 - 154. 6. Kennedy, G. et al. (2010) Biochemical and vascular aspects of pediatric chronic fatigue syndrome. Arch Pediatr Adolesc Med. 164: 817-23.

Additional Info

Recognizes an extracellular domain of the 55kD human TNF receptor 1, p55. No binding occurs to the 75kD TNF receptor. It is expressed weakly by monocytes and granulocytes. T9161-06C is routinely tested on human peripheral blood monocytes by Flow Cytometry. Species Crossreactivity: rabbit