Tumor Necrosis Factor Receptor 2, Soluble (sTNFR2)

TNF-RII is expressed on a variety of human tissues including lymphocytes, monocytes, granulocytes, fibroblasts, and endothelial cells. In addition, TNF-RII is released from neutrophils and monocytes after activation. TNF-RII, a member of TNF receptor family, binds to TNF-a and TNF-b. The cytoplasmic domain of TNF-RII interacts with TRAF1 and TRAF2.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilutions:\nELISA (Capture): Dilute in an appropriate coating buffer to a concentration of 1-5ug/ml and use 100ul to coat each well. Further dilutions may be made in medium or buffered solution with carrier protein, such as PBS with 0.1-1.0% BSA.\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nBALB/c splenocytes were fused with NSO mouse myeloma cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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