Tumor Necrosis Factor Receptor Associated Factor 3, (TRAF-3)\n

Human TRAF-3 is a signaling molecule that interacts with the cytoplasmic tails of CD40 and other TNF-receptor family members (1). The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the IL-1R/Toll and TNF receptor family to signalling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as a docking platform for a variety of regulators of these signalling pathways and are themselves often regulated at the transcriptional and posttranslational level (2). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500 \nImmunohistochemistry: 1:50 \nImmunocytochemistry: 1:100-1:250 \nFlow Cytometry: 1:20 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

T9161-56P

Size

100ul

Applications

FC, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. van Eyndhoven WG, et al. Mol Immunol 35(18):1189-206 1998 2. Wajant H, et al. Cell Signal 13(6):389-400, 2001 3. Ni CZ, et al. Proc Natl Acad Sci 97(19):10395-9, 2000

Additional Info

Recognizes human TRAF3.

Alternative Names

CD40 receptor-associated factor 1, CRAF1, CD40-binding protein, CD40BP, LMP1-associated protein, LAP1, CAP-1