TUMOR NECROSIS FACTOR RECEPTOR I (CD120a, CD120a antigen, FPF, MGC19588, p55, p55-R, p60, TBP1, TNFAR, TNF-R, TNFR1, TNF-R1, TNFR55, TNF-R55, TNFR60, TNFR-I, TNF-RI, TNF-R-I, Tumor necrosis factor receptor superfamily member 1A precursor) (FITC)

TNF-RI (~55-60 kD) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-R1 and TNF-R2, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-R1 is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-R2 is efficiently activated only by the membrane bound form of TNF-alpha. TNF-R1 signaling is initiated when trimeric TNF-alpha binds TNF-R1 receptors. Subsequent TNF-R1 trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-R1-deficient mice indicate that TNF-R1 mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways. \n\nApplications:\nSuitable for use in Immunoassay, Flow Cytometry, and Immunohistology (frozen). Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nImmunohistochemistry (frozen): 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nFor long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T9161-19

Size

100ug

Applications

ELISA, FC, IHC, IP

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

References

1. Bigini, P et al; Immunohistochemical localization of TNFa and its receptors in the rodent central nervous system. Methods Mol Med 2004, 98: 73 \n2. Mennini, T et al; Glial activation and TNFR-I upregulation precedes motor dysfunction in the spinal cord of mnd mice. Cytokine 2004, 25: 127 3. Ghezzi, P et al; Tumor necrosis factor as a pharmacological target. Methods Mol Med 2004, 98: 1

Additional Info

Recognizes the extracellular part and the soluble receptor of the mouse TUMOR NECROSIS FACTOR RECEPTOR I. Reactivity with soluble TNF-Receptor is inhibited by high conentrations of both mouse and human TNF-alpha.

Alternative Names

CD120b