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Twisted Gastrulation Protein, Recombinant, Human (TSG)

Cat no: T9188

Twisted Gastrulation Protein, Recombinant, Human (TSG)

Twisted Gastrulation Protein (TSG) is a secreted BMP binding protein structurally related to the BMP antagonists Chordin and Noggin. TSG can inhibit BMP activity by binding directly to BMP proteins, and can act either as a BMP4 agonist or antagonist (depending on the specific biochemical environment) by binding to the BMP4/Chordin complex. Recombinant human TSG is a 199 amino acid 22kD protein containing the BMP/TGFb binding portion of the full length TSG protein.\n\nBiological Activity:\nDetermined by its ability to neutralize BMP-6 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is < 2.0 mg/ml of TSG.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, carrier protein, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n\nCountry of Origin:\nUSA

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SPECIFICATIONS

Catalog Number

T9188

Size

10ug

Form

Supplied as a lyophilized powder in 10mM sodium acetate, pH6.0. Reconcentrate with dH2O, carrier protein to a concentration of 0.1-1mg/ml.

Purity

(same/more than) 98% by SDS-PAGE and HPLC analyses.

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