Macrolide antibiotics (including erythromycin and tylosin, etc) are macrocyclic lactones isolated first from Streptomyces spp. They are a very important class of antibacterial compounds widely used in medical and veterinary practice to treat respiratory diseases and enteric infections in cattle, sheep, swine and poultry. These compounds are highly active against a wide range of Gram-positive bacteria, Mycoplasma and Chlamydia. However, they are also employed as feed additives to promote animal growth. The use of these antibiotics may leave residues in edible tissues, causing adverse effects in consumers, e.g., allergic reactions in hypersensitive individuals or indirectly, problems through the induction of resistant strains of bacteria.
Applications:
Suitable for use in ELISA. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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