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Tyrosinase (TYR, CMM8, LB24-AB, Monophenol Monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor Rejection Antigen AB)

Cat no: T9230-08N

Tyrosinase (TYR, CMM8, LB24-AB, Monophenol Monooxygenase, OCA1A, OCAIA, SHEP3, SK29-AB, Tumor Rejection Antigen AB)

Tyrosinase (monophenol, dihydroxy-phenylalanine: oxygen oxidoreductase; E.C 1.14.18.1), a type I transmembrane glycoprotein involved in the regulation of pigmentation process in Humans. It is the rate-limiting enzyme involved in melanin biosynthesis and consists of a large catalytic luminal domain anchored to the membrane by a C-terminal transmembrane domain and a short cytosolic tail. Tyrosinase catalyzes the conversion of tyrosine to DOPA-quinone and then oxidization of 5, 6-dihydroxyindole to indole-5.6-quinone. Mutations in Tyrosinase cause tyrosinase negative type I oculocutaneous albinism (OCAI). Tyrosinase is a melanoma antigen and is an important target for anti-melanoma vaccine synthesis.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin): 1:25-1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMelanoma\n\nCellular Localization: \nCytoplasmic\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9230-08N

Size

500ul

Applications

IHC

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 0.05% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Popescu et.al JBC 280 (14) 13833-13840 (8 April, 2005). 2. Watabe et.al JBC 279 (9) 7971-7981 (27 February, 2004).

Additional Info

Recognizes human Tyrosinase.

Alternative Names

Tyrosinase (Oculocutaneous Albinism IA), EC=1.14.18.1

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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