Tyrosinase (TYR, LB24 AB, LB24-AB, Monophenol Monooxygenase, Oculocutaneous Albinism IA, OCA1A, OCAIA, SK29 AB, SK29-AB, Tumor Rejection Antigen AB)

Tyrosinase is the principal enzyme required for the synthesis of melanin pigment by melanocytes in mammals. It is also a marker of melanocyte differentiation, and is commonly expressed by malignant melanoma. Tyrosinase immunoreactivity studies in formalin-fixed, paraffin-embedded tissues have demonstrated that the T311 antibody is a specific, sensitive marker for the detection of melanocytic lesions. Immunoreactivity has also been shown to correlate inversely with clinical stage of melanoma progression. Because tyrosinase is recognized by cytotoxic T cells from melanoma patients, it has been proposed as an immunotherapeutic target for melanoma vaccines. The molecular mass of tyrosinase is 60-75kD. However, the processed, glycosylated forms of the protein found in melanosomes have been reported at 70-75kD. The T311 antibody identifies a cluster of proteins at 70-80kD, consistent with previous reports.\n\nApplications:\nSuitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1ug/ml\nWestern Blot: 1-5ug/ml\nNote: Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, prior to staining, is recommended for optimal immunohistochemical results with paraffin-embedded tissues.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9230-07

Size

100ug

Applications

IHC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liqud in PBS, pH 7.4, containing 0.1% sodium azide

P Type

Mab

Purity

Purified by Protein A chromatography

Isotype

IgG2a

Additional Info

Reacts with the human tyrosinase protein. On Western blots, it identifies a cluster of proteins ranging from 70-80kD and a minor band at 55kD in cell lines that are strongly reactive (ie SK-MEL-19). Reactivity has been confirmed with human SK-MEL-19, SK-MEL-13, and SK-MEL-37 melanoma cell lines.