Tyrosine hydroxylase is involved in the conversion of phenylalanine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons. Tyrosine hydroxylase is regularly used as a marker for dopaminergic neurons, which is particularly relevant for research into Parkinson's disease.
Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunofluorescence: 1:100
Western Blot: 1:1000
Immunohistochemistry: 1:100
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.
Storage and Stability:
For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.