Tyrosine Hydroxylase, phosphorylated (Ser19) (Tyrosine 3-hydroxylase, TH, TYH, DYT5b, DYT14, Tyrosine 3-monooxygenase)

Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies are useful markers for dopaminergic and noradrenergic neurons. Short-term regulation TH is accomplished by dynamic changes in the phosphorylation state of the enzyme. Four phosphorylation sites in TH have been identified (Ser8, Ser19, Ser31, and Ser40), all in the amino-terminal regulatory region. Phosphorylation of tyrosine hydroxylase Ser19 has been shown to increase both Ser40 phosphorylation and enzyme activity of tyrosine hydroxylase in intact cells.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:1000\nWestern Blot: 1:1000-1:2000\nImmunohistochemistry (frozen): 1:1000-1:2000\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nKCl stimulated or unstimulated PC-12 (10 min, 30mM)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

T9237-10T

Size

100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Witkovsky P, Gabriel R, Haycock, J W, and Meller E. 2002. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J. Chem. Neuroanat., 19:105 - 116. 2. Haycock JW, Lew J Y, Garcia-Espana A, Lee, K Y, Harada K, Meller E, and Goldstein M. 1998. Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis. 1998. J. Neurochem., 71:1670

Additional Info

Recognizes human Tyrosine Hydroxylase, phosphorylated at (Ser19).

Alternative Names

EC=1.14.16.2