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Tyrosine Hydroxylase, phosphorylated (Ser19) (Tyrosine 3-hydroxylase, TH, TYH, DYT5b, DYT14, Tyrosine 3-monooxygenase)

Tyrosine Hydroxylase, phosphorylated (Ser19) (Tyrosine 3-hydroxylase, TH, TYH, DYT5b, DYT14, Tyrosine 3-monooxygenase)

Cat no: T9237-10T

Supplier: United States Biological
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Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies are useful markers for dopaminergic and noradrenergic neurons. Short-term regulation TH is accomplished by dynamic changes in the phosphorylation state of the enzyme. Four phosphorylation sites in TH have been identified (Ser8, Ser19, Ser31, and Ser40), all in the amino-terminal regulatory region. Phosphorylation of tyrosine hydroxylase Ser19 has been shown to increase both Ser40 phosphorylation and enzyme activity of tyrosine hydroxylase in intact cells. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000 Western Blot: 1:1000-1:2000 Immunohistochemistry (frozen): 1:1000-1:2000 Optimal dilutions to be determined by the researcher. Positive Control: KCl stimulated or unstimulated PC-12 (10 min, 30mM) Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9237-10T
Reactivities: Human, Rat
Hosts: Rabbit
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=1.14.16.2
References: 1. Witkovsky P, Gabriel R, Haycock, J W, and Meller E. 2002. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J. Chem. Neuroanat., 19:105 - 116. 2. Haycock JW, Lew J Y, Garcia-Espana A, Lee, K Y, Harada K, Meller E, and Goldstein M. 1998. Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis. 1998. J. Neurochem., 71:1670
Additional info: Recognizes human Tyrosine Hydroxylase, phosphorylated at (Ser19).

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