Tyrosine Hydroxylase, phosphorylated (Ser31) (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Tyrosine hydroxylase (TH) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines dopamine and norepinephrine from tyrosine. TH is therefore a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. Phosphorylation of Ser31 can activate TH in vitro, and has been observed at this residue following NGF, phorbol ester, and diacylglycerol treatment of PC12 cells. Ser31 has been identified as a membrane depolarization-dependent and growth factor-sensitive phosphorylation site.\n\nApplications:\nSuitable for use in Western Blot, Immunofluorescence, and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunofluorescence: 1:1000\nImmunohistochemistry: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9237-10J

Size

100ul

Applications

IF, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150 mM NaCl, 0.1mg/ml BSA and 50% glycerol.

P Type

Pab

Purity

Supplied as a liquid in sequential chromatography on phospho- and nonphospho- peptide affinity columns

Additional Info

Specific for rat TH phosphorylated at Ser31. On Western blots, it identifies a single band at ~60kD. Reactivity has been confirmed with PC-12 cell lysates stimulated by okadaic acid.