Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Tyrosine Hydroxylase is a tetrameric enzyme composed of four 60-68kD subunits. TH catalyzes the hydroxylation of L-tyrosine to L-3,4-dihydroxylphenylalanine (L-dopa) in the brain and adrenal medulla. This is the initial and rate limiting step in the biosynthesis of catecholamines (dopamine, norepinephrine and epinephrine) that serve as neurotransmitters and hormones. TH is produced from a single gene. Its synthesis is regulated by transcriptional, translational and post-translational mechanisms. In most species, a single form of TH mRNA is produced and translated. Resulting in a single homotetrameric for of the protein. In the monkey, ape and human, TH RNA undergoes alternative splicing resulting in either two (monkey, ape) or four (human) isoforms of TH subunit protein. The four human isoforms are TH-1 (55.6kD), TH-2 (4 additional aa, 56kD), TH-3 (27 additional aa, 58.1kD) and TH-4 (31 addition aa, 58.5kD). Ony humans express all four isoforms. All other antrhropoids express on the TH-1 and TH-2 isoforms.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 using Chemiluminescence substrates.\nImmunoprecipitation (Native and denatured TH): 10-20ul antibody per 100-200ug of protein extracts.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9237-20

Size

50ul

Applications

IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, GP, NHP, Rabbit

Form

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1.

Additional Info

Recognizes rat N-terminal 1-20aa region and intact Tyrosine Hydroxylase. Species crossreactivity: mouse, human, bovine, monkey, sheep, guinea pig and rabbit. The monoclonal antibody has broad species crossreactivity.