Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine, epinephrine and norepinephrine. Therefore the regulation of the TH enzyme represents the central means for controlling the synthesis of these important catecholamines. TH has a large molecular diversity, resulting from differential splicing of its mRNA, which is tissue specific and might result in long term changes in activity of the enzyme and its availability of neurotransmitter at various synapses. \nThe presence of different DNA sequences at the TH locus confers susceptibility to various disorders of the brain including manic-depression and schizophrenia. Parkinson's disease is also considered a TH deficiency as low dopamine levels are a consistent neurochemical abnormality.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: PC-12 cells stimulated by okadaic acid\n\nCellular Localization: Can be used as a marker for dopaminergic and noradrenergic neurons.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9237-21C

Size

100ul

Applications

IF, IHC, IP, WB

Hosts

Rabbit

Form

10 HEPES [pH7.5], 150 mM NaCl, & 100ug/ml BSA with 50% glycerol

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Haavik, J. and Toska, K. Tyrosine hydroxylase and Parkinson's disease. Mol. Neurobiology 16(3) :285-309, 1998.\n2. Mallett, J. The TiPs/TINS lecture. Catecholamines from Gene Regulation to neuropsychiatric disorders. Trends in Pharmacological Science. 17(4): 129-135, 1996.\n3. Kumer SC, Vrana, KE, Intricate regulation of tyrosine hydroxylase activity and gene expression. Journal of Neurochemistry, 67(2) :443-462, 1996.\n4. Lewis DA, et al, Neuroscience 54: 477-, 1993\n5. Haycock, J. Anal. Biochemistry 181: 259-266, 1989.\n6. Haycock, J. Anal. Biochemistry 208: 397-399, 1993.\n7. Renfroe, J.B., et al. Brain Res. Bull. 13: 109 - 126, 1984.

Additional Info

Specific for tyrosine hydroxylase. Species Crossreactivity: Detects all mammalian forms of TH tested to date and some non-mammalian. Also detects some invertebrate forms of TH.