Tyrosine Hydroxylase (TH, TYH, Tyrosine 3 Hydroxylase, Tyrosine 3 Monooxygenase)

Tyrosine hydroxylase (TH) is a rate-limiting enzyme for catecholamine biosynthesis. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia, affective disorders, and Parkinsonism (1). Catecholamines that are produced by the catecholamine biosynthetic pathway, in which tyrosine hydroxylase catalyzes the initial, rate-limiting step, include dopamine, noradrenaline, and adrenaline. These 3 catecholamines are important neurotransmitters and hormones that regulate visceral functions, motor coordination, and arousal in adults. The TH gene becomes transcriptionally active in developing neuroblasts during midgestation of rodent embryos, before the onset of neurotransmission (2). Tyrosine hydroxylase catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-DOPA), the rate-limiting step in the biosynthesis of dopamine (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:100,000-1:200,000\nImmunocytochemistry: 1:100-1:250\nFlow Cytometry: 1:20\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

T9237-21K

Size

100ul

Applications

FC, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Superatant

Isotype

IgG

References

1. Knappskog PM, et al. Hum Mol Genet 4(7):1209-12, 1995 2. Zhou, QY et al. Nature 374:640-643, 1995 3. Kunugi H, et al. Am J Med Genet 81(2):131-3, 1998

Additional Info

Recognizes human Tyrosine 3-Hydroxylase. Species crossreactivity: mouse and rat.