Tyrosine hydroxylase (TH) is a rate-limiting enzyme for catecholamine biosynthesis. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia, affective disorders, and Parkinsonism (1). Catecholamines that are produced by the catecholamine biosynthetic pathway, in which tyrosine hydroxylase catalyzes the initial, rate-limiting step, include dopamine, noradrenaline, and adrenaline. These 3 catecholamines are important neurotransmitters and hormones that regulate visceral functions, motor coordination, and arousal in adults. The TH gene becomes transcriptionally active in developing neuroblasts during midgestation of rodent embryos, before the onset of neurotransmission (2). Tyrosine hydroxylase catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-DOPA), the rate-limiting step in the biosynthesis of dopamine (3).
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:100,000-1:200,000
Immunocytochemistry: 1:100-1:250
Flow Cytometry: 1:20
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Manufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
Quick Search
Products 
