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Tyrosine Hydroxylase (Tyrosine 3-hydroxylase, TH, TYH, DYT5b, DYT14, Tyrosine 3-monooxygenase)

Tyrosine Hydroxylase (Tyrosine 3-hydroxylase, TH, TYH, DYT5b, DYT14, Tyrosine 3-monooxygenase)

Cat no: T9237-10F

Supplier: United States Biological
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Tyrosine Hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies are useful markers for dopaminergic and noradrenergic neurons. Short-term regulation TH is accomplished by dynamic changes in the phosphorylation state of the enzyme. Four phosphorylation sites in TH have been identified (Ser8, Ser19, Ser31, and Ser40), all in the amino-terminal regulatory region. Cyclin-dependent kinase 5 (Cdk5) is involved in multiple aspects of neurotransmission in both post- and presynaptic compartments. Cdk5 has been described as the key enzyme for synthesis of dopamine and phosphorylates tyrosine hydroxylase at Ser31. Cdk5 also regulates ERK1/2-dependent phosphorylation of TH through the phosphorylation of mitogen-activated protein kinase kinase 1 (MEK1). Phospho-Ser31 TH levels have been found to increase in dopaminergic neurons of rats trained to chronically self-administer cocaine, suggesting that direct and indirect regulation of the phosphorylation state of the Ser31 site on TH by Cdk5/ERK1/2 may be involved in the neuroadaptive changes associated with chronic cocaine exposure. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:1000 Western Blot: 1:1000 Immunohistochemistry (frozen): 1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Okadaic treated PC-12 Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: T9237-10F
Reactivities: Rat
Hosts: Sheep
Applications: Immunofluorescence, Immunohistochemistry, Western Blot
Size: 100ul
Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
Alternative names: EC=1.14.16.2
References: 1. Witkovsky P, Gabriel R, Haycock, J W, and Meller E. 2002. Influence of light and neural circuitry on tyrosine hydroxylase phosphorylation in the rat retina. J. Chem. Neuroanat., 19:105 - 116. 2. Haycock JW, Lew J Y, Garcia-Espana A, Lee, K Y, Harada K, Meller E, and Goldstein M. 1998. Role of serine-19 phosphorylation in regulating tyrosine hydroxylase studied with site- and phosphospecific antibodies and site-directed mutagenesis. 1998. J. Neurochem., 71:1670
Additional info: Recognizes rat Tyrosine Hydroxylase. Species sequence homology: invertabrates and most mammals.

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