Tyrosine, phosphorylated (Phospho-Tyrosine) (HRP)

Tyrosine phosphorylation plays a key role in cellular signaling (1). In cancer, unregulated tyrosine\nkinase activity can drive malignancy and tumor formation by generating inappropriate roliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phosphotyrosine monoclonal antibodies provide exceptionally sensitive new tools of increased utility for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA-peptide: 1:1000\nWestern Blot: 1:2000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,\n0.1% Tween-20 at 4 degrees C with gentle shaking, overnight.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

T9230-04C

Size

200ul

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid in 136mM sodium chloride, 2.6mM KCl, 12mM sodium phosphate, pH7.4 dibasic, 2mg/ml BSA 50% glycerol. Labeled with horseradish peroxidase (HRP).

P Type

Mab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG1

References

(1) Schlessinger, J. (2000) Cell 103, 211-25. (2) Blume-Jensen, P. and Hunter, T. (2001) Nature 411,\n355-65. (3) Ward, S.G. et al. (1992) J Biol Chem 267, 23862-9. (4) Glenney, J.R. et al. (1988) J Immunol Methods 109, 277-85.

Additional Info

Recognizes Tyrosine, phosphorylated at (P-Tyr-100). Species Crossreactivity: All.