Tyrosine, phosphorylated (Tyr) (Agarose)

Since the original observations that protein tyrosine kinases (PTKs) play critical roles in oncogenic transformation (1,2), it has become clear that both protein tyrosine kinases and phospho-tyrosine phosphatases control a wide range of critical biological processes (3,4). Antibodies specific for phospho-tyrosine (5,6) have been invaluable reagents in these studies. Exceptionally sensitive new tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery. \n\nRecommended Dilution: Immunoprecipitation: 1:10; 20ul of 50% bead slurry to 200ul of cell lysate.\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

T9228-75A

Size

200ul

Applications

ELISA, IP

Hosts

Mouse

Conjugates

Agarose

Form

Supplied as a liquid in 1X Cell Lysis Buffer, 0.1mg/ml BSA, 50% glycerol. No preservative added.

P Type

Mab

Purity

Purified.

Isotype

IgG1

References

(1) Varmus, H. and Bishop, J.M. (1986) Cancer Surv. 5, 153

Additional Info

Useful for immunoprecipitation of tyrosine-phosphorylated proteins. Immobilized via the conjugation of carbohydrates to cross-linked agarose hydrazide beads. Does not crossreact with proteins phosphorylated on serine or threonine.