U1A, U2B snRNP (U1A, U2B Small Nuclear Ribonucleoprotein)

snRNPs (pronounced "snurps"), or small nuclear ribonucleoproteins, are RNA-protein complexes that combine with unmodified pre-mRNA and various other proteins to form a spliceosome, a large RNA-protein molecular complex upon which splicing of pre-mRNA occurs. The action of snRNPs is essential to the removal of introns from pre-mRNA, a critical aspect of post-transcriptional modification of RNA, occurring only in the nucleus of eukaryotic cells. The two essential components of snRNPs are protein molecules and RNA. The RNA found within each snRNP particle is known as small nuclear RNA, or snRNA, and is usually about 150 nucleotides in length. The snRNA component of the snRNP gives specificity to individual introns by "recognizing" the sequences of critical splicing signals at the 5' and 3' ends and branch site of introns. The snRNA in snRNPs is similar to ribosomal RNA in that it directly incorporates both an enzymatic and a structural role.\n\nApplications:\nSuitable for use in Immunoprecipitation, Immunofluorescence and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nImmunofluorescence: 1:5-1:10\nWestern Blot: 1:5-1:10\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHEp-2 cells\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS, 1% BSA, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U0005-60

Size

1ml

Applications

IF, IP, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder. Reconstitute with 1ml PBS, 1% BSA, 0.1% sodium azide.

P Type

Mab

Purity

Supernatant

Isotype

IgG1

References

1. Habets WJ et al. (1989) J Immunol 143:2560-2566.

Additional Info

Recognizes human U1A protein. Demostrates a minor crossreactivity with the U2B'' protein, contained in the U1 and U2 small nuclear ribonucleoprotein (snRNP) complexes.