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U2 Auxiliary Factor (U2AF65)

Cat no: U0800

U2 Auxiliary Factor (U2AF65)

U2 auxiliary factor (U2AF) is a highly conserved, non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site1. U2AF binds to a polypyrimidine tract at the 3' splice site. Binding of U2 snRNP with the help of U2AF and other auxiliary factors, produces the first stable complex in the mammalian spliceosome pathway\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-3ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U0800

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

P Type

Mab

Purity

Purified from mouse ascites fluid.

Isotype

IgG2a

References

1. Zamore D and Green M.R. (1991) EMBO J 10(1):207

Additional Info

Reacts with the 65kD human U2AF65 protein.

Read more on Supplier website

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

Reactivities

Hum, Mouse

More info

Applications

ELISA

Hosts

Mouse

More info
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