UBC 9 (Ubiquitin-Conjugating Enzyme 9)

UBCs are a family of proteins directly involved in ubiquitination of proteins and in the control of cellular processes through the targeting of key regulatory proteins for degradation UBC9 is a homologue of the class E2 ubiquitin-conjugating enzymes (UBCs) (1). UBC9 possesses a distinct electrostatic potential distribution that may provide possible clues to its ability to interact with other proteins. It was suggested that different UBCs may utilize catalytic mechanisms of varying efficiency and substrate specificity (2). UBC9 has been shown to catalyze conjugation of a small ubiquitin-like molecule-1 (SUMO-1) to a variety of target proteins. SUMO-1 modifications were implicated in the targeting of proteins to the nuclear envelope and certain intranuclear structures and in converting proteins resistant to ubiquitin-mediated degradation. UBC9 also interacts with the androgen receptor (AR), a member of the steroid receptor family of ligand-activated transcription factors. It has the ability to act as an AR co-regulator in a fashion independent of its ability to catalyze SUMO-1 conjugation. (3).\n\nApplications:\nSuitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5,000-1:10,000\nImmunohistochemistry: 1:100-1:250\nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

U0880-07

Size

100ul

Applications

FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1Yasugi T, et al. Nucleic Acids Res. 24(11):2005-10, 19962.Tong H, et al. J Biol Chem. 272(34):21381-7, 19973.Poukka H, et al. J Biol Chem. 274(27):19441-6, 1999

Additional Info

Recognizes human UBC9. Species crossreactivity: mouse and rat.