Ubc9, Recombinant, Human (Ubiquitin Conjugating Enzyme 9)

Human UBC9 Ubiquitin-like protein SUMO-1 conjugating enzyme is also called UBE2I. rHuUBC9 Mediates the conjugation of the ubiquitin-like protein SUMO-1 to a variety of proteins including RanGAP1, IkappaB alpha, and PML without the requirement of an ubiquitin ligase (E3).\n\nRecombinant Human ubiquitin-conjugating enzyme is a single, non-glycosylated, polypeptide chain having a molecular mass of 23kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nBiological Activity:\nIGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent proliferation of murine BALB\\C 3T3 cells (measured by H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10e6 U/mg. For most in-vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of ubiquitin-conjugating enzyme\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm\n2. Analysis by RP-HPLC, using a standard solution of UBC-9 as a Reference Standard.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U0880-06

Size

10ug

Form

Supplied as a liquid in 10mM Tris-HCl, pH 7.5, 100mM sodium chloride, 100mM imidazole, 0.5mM PMSF, 1mM DTT, 10% glycerol.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Wu PY, et al., (2004) EMBO J Dec 8;23(24):4876. 2. Houben K, et al., (2004) J Mol Biol Nov 19;344(2):513-26 3. Song L, et al., (2004) Parasitol Res Oct;94(3):227-32. 4. Verma S, et al., (2004) Mol Cell Biol Oct;24(19):8716-26 5. Winn PJ, et al., (2004) Structure (Camb) Sep;12(9):1563-74. 6. Merkley N, Shaw GS, (2004) J Biol Chem Nov 5;279(45):47139-47.