UBC9 (SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, Ubiquitin Carrier Protein I, Ubiquitin Carrier Protein 9, p18, UBE2I, UBC9, UBCE9) (PE)

Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumoylation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, IkBa, and PML without the requirement of an E3 ligase. Ubc9 is essential for nuclear architecture and chromosome segregation.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000 \nWestern Blot: 1:100-1:500\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze R-Phycoerythrin conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

U0880-03B-PE

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2. Contains ~0.09% sodium azide. Conjugated to R-Phycoerythrin (R-PE).

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1.Weldon, R.A. Jr., et al., Virology 314(1):62-73 (2003). 2.Lin, X., et al., J. Biol. Chem. 278(33):31043-31048 (2003). 3.Netzer, C., et al., Biochem. Biophys. Res. Commun. 296(4):870-876 (2002). 4.Sheng, W., et al., Protein Sci. 11(6):1482-1491 (2002). 5.Lin, D., et al., J. Biol. Chem. 277(24):21740-21748 (2002).

Additional Info

Recognizes human UBC9. Species Crossreactivity: mouse.