Ubc9 (Ubiquitin-Conjugating Enzyme 9)

Ubiquitin-conjugating enzyme 9 (Ubc9)1-3 is a component of the ubiquitin-mediated proteolytic SUMO (sentrin) pathway that targets proteins for degradation by the 26S proteasome, mediates endocytosis and directs protein subcellular localization.4,5 Ubc9 primarily mediates SUMOlation but not ubiquitinylation.6,7 An E3 ubiquitin ligase systematically transfers Ub and Ublike molecules from E2 conjugating enzymes to the targeted substrate.8 Ubc9, like other E2 family members, contains a conserved cysteine residue that is required for the thio ester formation between Ub-like proteins, and the E2 member, and it shares a conserved Ubc domain of approximately 16kD.9 More than 30 different vertebrate proteins are potential candidates for SUMOlation by Ubc9.10 Many Ubc9-interacting proteins contain a PEST sequence, a stretch of at least 12 amino acids rich in P, E, D, S, and T amino acid residues.10-13 Substrates for Ubc9 include transcription factors E12 and E47 and mitotic regulators RanBP2 and RanGAP1, indicating that Ubc9 may regulate various cellular processes including cell cycle progression and differentiation.14,15 Many Ubc9-interacting proteins have been implicated in the maintenance of vertebrate genome integrity, including the tumor suppressor protein p53,16 the promyelocytic leukemia protein (Pml),17 the poly(ADP-ribose) polymerase PARP18 and the homologous recombination proteins Rad51/52.13,16,19 Ubc9 is predominantly present in the nucleus, and at the nuclear pore complex.10, 20-22 Ubc9

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

U0880-03

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Epitope-affinity purified from rabbit antiserum.

Isotype

IgG

References

1. Hateboer G, et al. (1996) J Biol Chem 271:25906-25911. 2. Seufert W, et al. (1995) Nature 373:78-81. 3. Yasugi T and Howley PM. (1996) Nucleic Acids Res 24:2005-2010. 4. Hochstrasser M. (1996) Cell 84:813-815. 5. Wang ZY, et al. (1996) J Biol Chem 271:24811-24816. 6. Johnson ES and Blobel G. (1997) J Biol Chem 272:26799-26802. 7. Schwarz SE, et al. (1998) PNAS 95:560-564.\n8. Jentsch S. (1992) Annu Rev Genet 26:179-207. 9. Okuma T, et al. (1999) Biochem Biophys Res Commun 254:693-698. 10. Melchior F. (2000) Annu Rev Cell Dev Biol 16:591-626. 11. Kim YH, et al. (1999) PNAS 96:12350-12355. 12. Rechsteiner M and Rogers SW. (1996) Trends Biochem Sci 21:267-271. 13. Saitoh H, et al. J Biol Chem (2002) 277:4755-4763. 14. Huggins GS, et al. (1999) J Biol Chem 274:28690-28696. 15. Saitoh H, et al. (1998) Curr Biol 8:121-124. 16. Shen Z, et al. (1996) Genomics 37:183-186. 17. Duprez E, et al. (1999) J Cell Sci 112(3):381-393. 18. Masson M, et al. (1997) Gene 190:287296. 19. Kovalenko OV, et al. (1996) PNAS 93:2958-63. 20. Firestein R and Feuerstein (1998) N. J Biol Chem 273:5892-5902. 21. Lee GW, et al. (1998) J Biol Chem 273:6503-6507. 22. Rodriguez MS, et al. (2001) J Biol Chem 276:12654-12659.

Additional Info

Reacts with human, mouse, rat and golden hamster ubiquitin-conjugating enzyme 9 (Ubc9) protein. On Western blots, it identifies the target band at ~18kD. A band at ~13kD is observed in mouse spleen homogenates. Reactivity has been confirmed HEK293, HepG2, HL-60 cell lysates and mouse spleen homogenates.