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UbcH6, Active, Recombinant, Human, His-Tag (UBE2E1, Ubiquitin-conjugating Enzyme E2 E1, UbcH6, Ubiquitin Carrier Protein E1, Ubiquitin-protein Ligase E1)

Cat no: 137388

UbcH6, Active, Recombinant, Human, His-Tag (UBE2E1, Ubiquitin-conjugating Enzyme E2 E1, UbcH6, Ubiquitin Carrier Protein E1, Ubiquitin-protein Ligase E1)

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.\n\nSource:\nRecombinant corresponding to aa2-end from human UbcH6, fused to His-tag at N-terminal, expressed in E. coli.\n\nMolecular Weight:\n~22kD\n\nApplications: \nSuitable for for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

137388

Size

100ug

Form

Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 200mM imidazole, 20% glycerol, 3mM DTT.

Purity

Purified (~65%)

References

1. Sommer, T., et al. 1993. Nature 365 (6442): 176-9. 2. Yang, M. et al. 1997. J. Biol. Chem. 272: 1970-1975.

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