Ube2 J1 (Ubiquitin-Conjugating Enzyme E2 J1)

Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a \ndistinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response. \n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:1,000-1:5,000\nWestern Blot: 1:200-1:2,000\nImmunoprecipitation: 1-2ug\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U1000-87B

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Lenk,U., Yu,H., Walter,J., Gelman,M.S., Hartmann,E., Kopito,R.R. and Sommer,T. (2002) A role for mammalian Ubc6 homologues in ER-associated protein degradation. J. Cell Sci. 115:3007-3014. \n2.Lester,D., Farquharson,C., Russell,G. and Houston,B. (2000) Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. Biochem. Biophys. Res. Commun. 269(2):474-480. 3Jentsch, S. and Pyrowolakis, G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-342.

Additional Info

Recognizes human. Species Sequence Homology: mouse and rat.