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Ube2 J1 (Ubiquitin-Conjugating Enzyme E2 J1)

Ube2 J1 (Ubiquitin-Conjugating Enzyme E2 J1)

Cat no: U1000-87B

Supplier: United States Biological
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Ube2j1 and Ube2j2 are homologs of the yeast ubiquitin-conjugating enzyme UBC6, which catalyzes the covalent attachment of ubiquitin to other proteins. These proteins constitute a distinct family of ubiquitin-conjugating enzymes sharing a conserved non-canonical active site sequence and a C-terminal transmembrane domain. By analogy with yeast UBC6, Ube2j1 and Ube2j2 are localized to the endoplasmic reticulum and seem to function in the selective degradation of misfolded membrane proteins and in general mediation of the stress response. Applications: Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:1,000-1:5,000 Western Blot: 1:200-1:2,000 Immunoprecipitation: 1-2ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: U1000-87B
Hosts: Rabbit
Applications: ELISA, Immunoprecipitation, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography.
References: 1.Lenk,U., Yu,H., Walter,J., Gelman,M.S., Hartmann,E., Kopito,R.R. and Sommer,T. (2002) A role for mammalian Ubc6 homologues in ER-associated protein degradation. J. Cell Sci. 115:3007-3014. 2.Lester,D., Farquharson,C., Russell,G. and Houston,B. (2000) Identification of a family of noncanonical ubiquitin-conjugating enzymes structurally related to yeast UBC6. Biochem. Biophys. Res. Commun. 269(2):474-480. 3Jentsch, S. and Pyrowolakis, G. (2000) Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 10(8):335-342.
Additional info: Recognizes human. Species Sequence Homology: mouse and rat.

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