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UBE2D3, Recombinant, Human, aa1-149, His-tag (Ubiquitin-conjugating Enzyme E2 D3, Ubiquitin-protein Ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating Enzyme E2-17kD 3, Ubiquitin-conjugating Enzyme E2(17)KB 3, UBCH5C)

Cat no: U0880-08D

UBE2D3, Recombinant, Human, aa1-149, His-tag (Ubiquitin-conjugating Enzyme E2 D3, Ubiquitin-protein Ligase D3, Ubiquitin carrier protein D3, Ubiquitin-conjugating Enzyme E2-17kD 3, Ubiquitin-conjugating Enzyme E2(17)KB 3, UBCH5C)

UBE2D3, the modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.\n\nSource:\nRecombinant corresponding to aa1-149 from human UBE2D3, fused to His-tag at N-terminal expressed in E.coli.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MLSNRKCLSK ELSDLARDPP AQCSAGPVGD DMFHWQATIM GPNDSPYQGG VFFLTIHFPT DYPFKPPKVA FTTRIYHPNI NSNGSICLDI LRSQWSPALT ISKVLLSICS LLCDPNPDDP LVPEIARIYK TDRDKYNRIS REWTQKYAM\n\nMolecular Weight:\n~19.1kD (MALDI-TOF)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

U0880-08D

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 40% glycerol, 0.15M sodium chloride, 1mM DTT.

Purity

~90% (SDS-PAGE)

References

1. Gonen H., et al. (1999) J. Biol. Chem. 274:14823-14830. 2. Murata S., et al. (2001) EMBO Rep. 2:1133-1138.

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