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UBE2F (NCE2, MGC18120, NEDD8 or Ubiquitin Conjugating Enzyme E2F putative)

UBE2F (NCE2, MGC18120, NEDD8 or Ubiquitin Conjugating Enzyme E2F putative)

Cat no: U0880-10B

Supplier: United States Biological
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UBE2F (Ubiquitin-conjugating enzyme E2F), a ubiquitously expressed enzyme and member of the ubiquitin-like E2 conjugating enzyme family, which acts as a specific NEDD8 conjugating enzyme, by accepting NEDD8 from the Uba3-NAE1 (APP-BP1/Uba3) E1 complex. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: Peptide ELISA Titer: 1:16,000 Western Blot: 0.05-0.2ug/ml detects ~20kD in Mouse Liver lysates (calculated MW of 21.1kD according to Human NP_542409.1 and to Mouse NP_080730.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: U0880-10B
Reactivities: Human, Mouse
Hosts: Goat
Applications: ELISA, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
P type: Pab
Isotype: IgG
Purity: Purified by peptide affinity chromatography.
Alternative names: NEDD8 conjugating enzyme, NCE2, MGC18120, ubiquitin-conjugating enzyme E2F (putative), UBE2F
References: 1. Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol. Cell 33 (4), 483-495 (2009).
Additional info: Recognizes UBE2F. Species crossreactivity: mouse. Species sequence homology: human, rat, canine and bovine.

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